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American Barrelfish


Polymerase chain reaction (PCR).  Step1: Solution is heated to 95°C to denature (unzip) the two strands of the target DNA (A).  Step 2:  Solution is cooled to ~55°C to allow the primers to anneal (bind) to the ends of the DNA strands (B).  Step 3:  Solution is reheated to ~75°C to allow TAQ polymerase to synthesize complementary copies of each strand. 


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