WEBVTT

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One of the main objectives is
to look at connectivity of

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these species throughout more
than just the gulf. So we're

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looking at connectivity over
the entire Pulley Ridge, and

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then how connected these
populations say in the Dry

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Tortugas or those shallow
populations in the Keys. It's

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becoming a bigger issue for
management especially to

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understand how a population
actually functions. And to be

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able to manage it effectively.
A typical day after we get up

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and have our breakfast, is to
go out and make sure their

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sampling bags are ready to go,
we have these clamshell mesh

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bags inside of which I put
individual ziplock bags that

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are on zip ties that are all
labeled so we know where we

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got all those samples from.
After I get their bags ready

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to go, I start setting up my
workstation-which is a big

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plastic tub with a cutting
board in it. It allows me to

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frag out the corals, to cut
samples off of the fish.

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Preserves anything, keeps all
my tubes right next door to

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all my preservatives. Makes
sure everything gets done

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quickly and without messing up
all boat. So were out here on

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Pulley Ridge for a big project
that the University of Miami

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got a couple of years ago. The
dives themselves have been in

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about 220, averaging about 225
feet of water. Our bottom

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times are anywhere from 15 to
30 minutes, which puts us in

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the water somewhere between 90
and 115 minutes. The sampling

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itself has gone pretty well, a
lot of what we are doing is

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being opportunistic. We get
what we find. When we get on

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the bottom, we have to do a
very quick assessment of the

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bottom-and then we collect we
have. If we see a lot of fish,

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we collect as many fish as we
can. If we see a lot of

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corals, were collecting
corals. If we see sponge and

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algae-we're going after those.
We've done pretty well on this

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trip, we've been able to
collect as many fish as we

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can, so that's a good thing.
We're still working on some

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coral, we're still working on
some algae. Another thing

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we've been able to accomplish
on this trip is physical

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oceanography moorings. We were
able to find, first of all,

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the three moorings. But then
also recover the instruments

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that were on there, and
replace those instruments with

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new updated instruments. We're
diving off of the M/V Spree,

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which is a great platform for
this type of diving. And we're

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able to do this type of
project because of the

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assistance of the Florida
Aquarium and University of

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Puerto Rico divers. Our
profiles are pretty much a 20

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minute bottom time on average.
So what happens is we'll hit

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the surface of the water,
we'll drop down about 20

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feet-each person will get with
their buddy and do a bubble

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check to make sure that their
rebreathers are working. Once

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everybody is checked out and
everything is working, then

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we'll do an ascent down to the
bottom. It usually takes us

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about 3 minutes to get to the
bottom, at which point we

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start working. We do a very
quick assessment, once we

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start-once we understand what
the bottom is like, then we

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start collecting. We stay on
the bottom for about 17

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minutes at which point we get
together. 17 minutes we put

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all the samples in one bag and
we send that bag to the

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surface. Once that bag has
left our hands then we start

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our ascent. Our ascent moves
up to 150 feet, where a couple

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of us shoot whats called a SMB
a surface marker buoy. This

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allows the boat to see where
we are. Then we work our way

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along that surface marker buoy
up to the surface.

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Decompression phase of the
dive takes anywhere from 60 to

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90 minutes depending on how
long we've actually been on

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the bottom. So this cruise is
all about adding more samples

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to the ones we collected last
year. We're hitting a lot of

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the same sites as last
year-the ones that we knew

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were going to produce a lot of
different species. So know we

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are just bulking up those
sample sizes, especially for

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these molecular analysis. The
larger the sample size-the

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better. You are going to get a
much stronger analysis,

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especially when you are
looking at something like

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connectivity of these
populations to other areas.