WEBVTT 1 00:00:09.333 --> 00:00:21.333 What's really cool about this site is that there's a really 2 00:00:12.600 --> 00:00:18.333 tall chimney structure, and yeah- they keep saying it's the largest 3 00:00:18.333 --> 00:00:25.100 biological iron structure. It was on the back art of the mariana, 4 00:00:25.100 --> 00:00:33.000 So we sampled the bottom, and the middle, and the top of this 5 00:00:33.000 --> 00:00:37.100 structure. We got scoop samples, we got slurp samples, and we got 6 00:00:37.100 --> 00:00:39.967 biomat samples. The biomat sampler is that cassette sampler, 7 00:00:39.967 --> 00:00:44.067 So we get that really fine scale, community structure. So, the ones 8 00:00:44.067 --> 00:00:47.700 that settle out are - have a lot more mineral particles in them. 9 00:00:47.700 --> 00:00:51.333 And so those tend to be less active. and so the fluffy stuff 10 00:00:51.333 --> 00:00:54.467 is where we think we have the active zetaproteo bacteria. See? 11 00:00:54.467 --> 00:00:59.233 Not fluffy. Not fluffy enough at all. and this is fluffy. We want 12 00:00:59.233 --> 00:01:04.233 fluffy. So what I'm going to do when I get home is going to 13 00:01:04.233 --> 00:01:07.733 compare these samples, find the ones that are the most zeta 14 00:01:07.733 --> 00:01:12.167 enriched, so determine the zeta percentage of the samples and 15 00:01:12.167 --> 00:01:15.867 then I will do metageonomics. Which means I will sequence all 16 00:01:15.867 --> 00:01:19.900 of it. And then from that I will determine what genes are 17 00:01:19.900 --> 00:01:23.700 responsible for carbon fixation, umm how are electrons getting 18 00:01:23.700 --> 00:01:26.433 transferred, so how are they making their energy, how are they 19 00:01:26.433 --> 00:01:28.433 making a living.