WEBVTT 1 00:00:02.006 --> 00:00:07.541 So this is a, let’s see if I get this right, Zetaproteobacteria. Correct. 2 00:00:13.120 --> 00:00:22.460 We’re focusing our efforts quite a bit on iron microbiology and we’re finding there’s an awful lot of iron that’s getting put into the oceans, 3 00:00:23.258 --> 00:00:26.535 basically fertilizing the oceans, that’s coming from hydrothermal vents. 4 00:00:27.593 --> 00:00:31.040 We’ve got our intact mat at the bottom here. 5 00:00:31.792 --> 00:00:37.179 Craig Moyer and his group are very interested in the iron-oxidizing microbial mats. 6 00:00:37.923 --> 00:00:39.505 There’s a little piece of intact mat. 7 00:00:40.295 --> 00:00:43.619 They’re kind of fluffy deposits that we see on the seafloor. 8 00:00:44.374 --> 00:00:45.871 It’s not that little. This is big. 9 00:00:46.627 --> 00:00:51.261 The iron mats are made up of what’s called iron ferrihydrite, primarily. 10 00:00:51.759 --> 00:00:53.345 They can also have some clays in them. 11 00:00:53.872 --> 00:01:02.510 They have a lot of polysaccharides, or microbial snot, and then there’s the cells that are actually doing a lot of the chemical transformations. 12 00:01:03.292 --> 00:01:03.777 That one’s kind of cool. 13 00:01:03.877 --> 00:01:12.394 So we brought up big chunks of the microbial mat, which we hope are intact, and then with the scalpel, 14 00:01:12.891 --> 00:01:17.761 we try to like, peel back layers, kind of, to show if there’s any directionality or orientation that we can see just by looking at it. 15 00:01:18.306 --> 00:01:22.682 I think it, like, this is the outside, right here, and they’re all coming, like, up. 16 00:01:23.264 --> 00:01:31.143 The bacteria are oxidizing iron, but what that’s basically doing is taking iron from the soluble form and converting it to a solid form. 17 00:01:31.926 --> 00:01:35.599 This was crotched by Margo Heygood as a gift for me for my master’s degree. 18 00:01:36.400 --> 00:01:39.094 So these are the cells of the Zetaproteobacteria on the end. 19 00:01:40.478 --> 00:01:42.258 So that bacteria is just this part? 20 00:01:42.757 --> 00:01:43.012 Yes. 21 00:01:43.560 --> 00:01:45.099 And everything else is just iron oxide? 22 00:01:45.901 --> 00:01:48.977 Yeah. And so all this is the iron oxide that the cells are producing. 23 00:01:49.478 --> 00:01:52.098 So this is not life size. 24 00:01:52.596 --> 00:01:58.478 No. But it’s a bit to scale. And it’s meant to represent what we know about the Zetaproteobacteria. 25 00:01:59.324 --> 00:02:04.247 Zetaproteobacteria seem to dominate all the iron systems in and around hydrothermal vents. 26 00:02:04.994 --> 00:02:10.356 They’re one of the main iron oxidizers that occur in the microbial mats in marine systems. 27 00:02:10.937 --> 00:02:12.650 The different bacteria make cool structures. 28 00:02:13.148 --> 00:02:15.765 Some of them twist, the iron comes out twisted, we call it a stalk. 29 00:02:16.309 --> 00:02:19.649 Sometimes it’s like a tube. We call that a sheath. 30 00:02:20.732 --> 00:02:25.398 So you can tell something about what was living there, like, based on what they make, what kind of structures they make. 31 00:02:25.896 --> 00:02:29.257 So the cell uses the iron oxides to move through the environment. 32 00:02:29.815 --> 00:02:30.312 Oh really. 33 00:02:30.813 --> 00:02:31.812 And it’s twisting as it’s doing that. 34 00:02:32.308 --> 00:02:38.400 And then as it’s gaining energy, growing larger, then it divides, and that’s what’s represented by… 35 00:02:38.921 --> 00:02:41.063 Oh, so these two guys used to be one, and now they’re two? 36 00:02:41.591 --> 00:02:41.847 Exactly. 37 00:02:42.395 --> 00:02:43.674 That is crazy. 38 00:02:44.182 --> 00:02:49.149 And when we look at the mats, you can tell which direction the mat was formed in by looking for these division points. 39 00:02:49.673 --> 00:02:50.175 Wow. 40 00:03:01.567 --> 00:03:11.092 When you see these nice mats on the bottom, they’re not just all microbiology, but it’s really the history of the microbes that have been there before them, 41 00:03:11.599 --> 00:03:14.150 as well as the ones that are actually growing right now. 42 00:03:15.661 --> 00:03:20.398 It is good in the lab where it’s a bit air conditioned, but it doesn’t quite fit around as a scarf.